The role of Rhipicephalus sanguineus sensu lato saliva in the dissemination of Rickettsia conorii in C3H/HeJ mice.

Animal models have been developed for the study of rickettsial pathogenesis. However, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. To address this, we analysed the role of tick saliva in the transmission of Rickettsia conorii (Rickettsiales: Rickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible C3H/HeJ mice with this Rickettsia, and infesting one group with nymphal Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) ticks. Quantification of bacterial loads and mRNA levels of interleukin-1β (IL-1β), IL-10 and NF-κB was performed in C3H/HeJ lung samples by real-time quantitative polymerase chain reaction (PCR) and real-time reverse transcriptase PCR, respectively. Lung histology was examined to evaluate the pathological manifestations of infection. No statistically significant difference in bacterial load in the lungs of mice was observed between these two groups; however, a statistically significant difference was observed in levels of IL-1β and NF-κB, both of which were higher in the group inoculated with rickettsiae but not infected with ticks. Lung histology in both groups of animals revealed infiltration of inflammatory cells. Overall, this study showed that i.d. inoculation of R. conorii caused infection in the lungs of C3H/HeJ mice and tick saliva inhibited proinflammatory effects.

Oral food perception and polyphenol-rich foods acceptance - the importance of knowing individuals saliva characteristics for promoting consumption

Polyphenols are widely present in fruits, vegetables, cereals and beverages. Their study gained scientific interest because of their beneficial effects on health. Although there is currently no official dietary recommendation for polyphenol intake, health professionals recommend the consumption of 5-8 daily portions of fruits and vegetables. This is not always achieved and, despite possible causes associated to practical schedule difficulties, the aversive bitter and astringent sensations associated to polyphenols may also lead to avoidance. As such, a better understanding on mechanisms responsible for differences among people, in polyphenol oral perception, is needed for promoting healthier choices. Saliva has been linked to polyphenol consumption. We have previously observed, in animal models, changes in salivary proteome induced by tannin-enriched diets. Moreover, differences in astringency perception were attributed to differences in salivary protein composition. In a recent experiment, we observed differences among individuals with dissimilar tannic-acid perception: people with high sensitivity for the oral sensations elicited by tannins have higher amounts of salivary cystatins and lower capacity to maintain their levels after tannic-acid ingestion. Additionally, and similarly to previous studies, salivary amylase was observed to be involved in tannin perception. In this presentation, oral cavity characteristics influencing the perception of polyphenol-containing foods will be discussed.

Alterações do comportamento em adolescentes: uma abordagem em enfermagem

Com este trabalho pretende-se realçar que os adolescentes passam por determinadas fases ou estádios da sua vida, em que se deparam com determinadas dificuldades ou até mesmo, incapacidades para levar a cabo a realização de determinadas necessidades; e que por isso mesmo, estes momentos podem vir a representar a oportunidade de crescimento da personalidade, mas ao mesmo tempo, o perigo de crescente vulnerabilidade ao distúrbio mental. Este relatório, expressa a preocupação com a problemática, analisa reflexivamente e descreve o trabalho desenvolvido na área da saúde mental e psiquiátrica por um enfermeiro, na Clínica da Juventude ao longo de seis meses, através da implementação do que se preconiza ser um Processo de Cuidados em Enfermagem (i.e., avaliação, diagnóstico, planeamento, intervenção e avaliação final), no acompanhamento de adolescentes com alterações do comportamento. O instrumento usado para avaliação diagnóstica foi a entrevista semiestrutura e semidireccionada, o planeamento e as intervenções variaram de acordo com a individualidade de cada adolescente e as suas necessidades particulares. Os resultados de uma forma geral demonstraram que o acompanhamento dos jovens com alterações do comportamento deve iniciar-se o mais precocemente possível, deve incidir na identificação das principais dificuldades ou necessidades e não nos diagnósticos médicos. O adolescente, deve também, ser abordado o mais holísticamente possível e nos diferentes setores onde se mobiliza. Conclui-se que a intencionalidade terapêutica deve recair no acompanhamento do adolescente, no sentido, de o tornar mais autónomo, capaz de assumir responsabilidades, tomar decisões, que cresça e se transforme num adulto autoconfiante e realizado; ABSTRACT: This work aims to emphasize that teenagers go through certain phases or stages of its life, when faced with certain difficulties and even disabilities to carry out the execution of particular needs, and that therefore, these moments may come to represent the opportunity for growth of personality, but at the same time, the danger of increasing vulnerability to mental disorder. This report expresses concern with the issue, reflective analysis and describes the work in the area of mental health and psychiatric care by a nurse in the Youth Clinic for six months, by implementing what it calls a procedure to be Care Nursing (ie, assessment, diagnosis, planning, intervention and final assessment), monitoring of adolescents with behavioral changes. The results generally showed that the monitoring of young people with behavioral changes should be initiated as early as possible, should focus on identifying the main difficulties or needs and not on medical diagnostics. The teen should also be approached holistically as possible and in different sectors where it is mobilized. We conclude that the intention must lie in therapeutic monitoring of the adolescent, in order, making it more autonomous, able to take responsibility, make decisions, to grow and become an adult self-confident and accomplished.